Table 1. Binding of native HDL and acro-HDL to SR-BI and selective uptake of COE.

		Binding to SR-BI(ng HDL protein/mg cell protein) ± SEM		Uptake of HDL-COE(ng HDL-COE protein/mg cell protein) ± SEM
		HDL	Acro-HDL	HDL	Acro-HDL
Lipoproteinconcentration(μg/mL)	2.5	27.5 ± 3.6	29.3 ± 2.6	179.2 ± 19.8	147.8 ± 21.5
	5	54.1 ± 7.0	54.7 ± 4.1	324.0 ± 38.0	260.5 ± 35.4
	10	88.7 ± 9.2	108.9 ± 9.7 ***	551.6 ± 64.1	591.3 ± 61.6
	25	117.3 ± 7.6	125.7 ± 7.6	1149.6 ± 133.1	963.5 ± 105.8 *
	50	168.9 ± 5.8	205.4 ± 13.7 **	956.5 ± 153.6	1085.2 ± 164.9
Time (min)	5	18.0 ± 1.2	15.9 ± 1.7	5.3 ± 0.4	5.0 ± 0.4
	10	27.3 ± 1.8	24.2 ± 1.8	9.1 ± 0.7	7.5 ± 0.7 *
	20	35.1 ± 2.2	42.3 ± 0.9 **	16.0 ± 1.3	15.6 ± 1.5
	30	37.4 ± 2.4	39.4 ± 3.5	17.7 ± 2.7	18.0 ± 2.8
	45	44.3 ± 4.3	53.5 ± 2.3 *	30.3 ± 5.0	21.4 ± 2.2
	90	52.0 ± 4.1	59.7 ± 3.9 **	46.3 ± 7.8	37.1 ± 6.2 *
	180	45.8 ± 7.7	64.7 ± 3.1 *	74.3 ± 15.3	68.1 ± 10.6

COS-7 cells transiently transfected with empty vector (pcDNA3) or human SR-BI were incubated with native- or acrolein-modified [¹²⁵I]/[³H]-COE-labeled HDL. Binding of radiolabeled HDL/acro-HDL to SR-BI and selective uptake of HDL-COE were measured as a function of ligand concentration (for 1.5 hours at 37°C) or incubation time (ligand concentration of 10 μg/mL at 37°C). Values represent the mean ± SEM of at least four independent experiments, each performed in triplicate. Data sets are presented following subtraction of empty vector values. Statistical analyses were determined by paired t-test.

*p<0.05,

**p<0.01

***p<0.001