Fig 3. Effect of a reducing agent on the ability of Env variants to mediate infection and cell-cell fusion.
(A). Normalized amounts of recombinant luciferase-expressing HIV-1 pseudotyped with the wt and mutant HIV-1BG505 ΔCT Envs were spin-inoculated onto Cf2Th-CD4/CCR5 cells before adding increasing concentrations of DTT (0–5 mM). (B). The ability of the indicated Env variants to mediate cell-cell fusion in the presence of increasing concentrations of DTT was evaluated. The data shown are from a single experiment and are representative of results from at least three independent experiments, performed in quadruplicate.