Fig 6. EgrCBF and EgrDREB2 transcript abundance in leaves of E. gunnii x E. dalrympleana plants grown for 8h under 4°C cold (white) or control conditions (black).

Gene expression levels of EgrCBF (C1 to C17) and EgrDREB2 (D2-1 to -6) were quantified on leaves from cold-treated Eucalyptus plants. The experimental values from RT-qPCR (conducted on three biological replicates) were normalized using five reference genes and the mean values (n = 3) were analyzed for significance using the statistical T-test (p value < 0.05). The resulting histograms represent Log₁₀ of relative transcript abundance of the CBF and DREB2 in control or cold conditions. * means a significant 2-fold rate increase in gene expression (cold/control)