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. 2015 Apr 7;10(4):e0122095. doi: 10.1371/journal.pone.0122095

Fig 1. Resistance to inactivation of the lectin activity of Blad-oligomer.

Fig 1

Blad-oligomer was purified, incubated for 10 min at 0°C in the presence of water (control, lanes 1 and 5), 4 N HCl (lane 2), 8 N HCl (lane 3) and 12 N HCl (lane 4). The protein samples were subsequently analysed by SDS-PAGE and stained for total protein (50 μg per lane; A) or blotted onto a membrane and probed with anti-blad oligomer antibodies (15 μg per lane; B) or with affinity purified, anti-ubiquitin antibodies (50 μg per lane; C). Molecular masses of standards are indicated in kDa.