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. Author manuscript; available in PMC: 2015 Jun 9.
Published in final edited form as: Nat Commun. 2014 Jun 9;5:4092. doi: 10.1038/ncomms5092

Figure 1. BCR-activated B cells induce maturation of DCs.

Figure 1

(a) Experimental design. Immature DCs were cultured for 48 h in the medium containing granulocyte macrophage colony-stimulating factor and IL-4 alone (DCctrl), or co-cultured at 1:1 ratio with CD19 + B cells that were either in resting phase (DCRest-B) or directly activated in DC–B cell co-culture via BCR stimuli (DCBCR-B). Phenotypic analysis of CD20-negative cells was done by flow cytometry. (b) BCR-activated B cells enhance the expression (% positive cells and mean fluorescence intensity, MFI) of DC activation markers and co-stimulatory molecules. (c) BCR-activated B cells upregulate expression of HLA-DR and CD40 on DCs. (d) Modulation of expression of CD11c, DC-SIGN, CD1a and CD206 on DCs by BCR-activated B cells. Representative plot and mean±s.e.m. of data from 10 to 24 donors. (e) Immature DCs were co-cultured with increasing number of B cells in the presence of BCR stimuli for 48 h and expression (% positive cells and MFI) of CD83 and CD86 was analysed on CD20-negative cells. Mean±s.e.m. of data from four donors. *P<0.05; **P<0.01; ***P<0.001 by one-way analysis of variance test.