Attraction and invasion of Meloidogyne incognita J2 to potato roots with and without 16D10i-2 RNAi transgene. A. Number of infective J2 touching the 10-mm terminal end of potato roots with and without 16D10i-2 RNAi transgene at 6 hr after inoculation. B. Number of parasitic J2 invading potato roots with and without 16D10i-2 RNAi transgene at 4 days after inoculation (DAI). Each bar represents the mean of 15 root tips per independent line with SE. Student’s t-test did not indicate significant differences (P > 0.05). 82-4, PA99N82-4 advanced breeding line; RB, wild type ‘Russet Burbank’; E34, empty vector control; D21, 16D10i-2 transgenic line D21. C–F. Attraction of M. incognita infective J2 to representative root tips at 6 hr after inoculation in potato lines 82-4, RB, E34, and D21, respectively. G–J. Invasion of M. incognita parasitic J2 in representative roots at 4 DAI after acid fuchsin staining in potato lines 82-4, RB, E34, and D21, respectively. Scale bar = 100 µm.