Table 1. PCR primers and annealing temperatures used to amplify mitochondrial and nuclear markers in this study.

Gene	Primer	Sequence (5'–to–3')	PCR steps §	T A (annealing temperature,°C)	Reference
cytb	L14725	GAYTTGAARAACCAYCGTTG	Single PCR	48	Hrbek et al. [106]
	H15982	CCTAGCTTTGGGAGYTAGG	Single PCR	48	Hrbek et al. [106]
cox1	FISH-F1	TCAACCAACCACAAAGACATTGGCAC	Single PCR	48–49	Ward et al. [107]
	FISH-R1	TAGACTTCTGGGTGGCCAAAGAATCA	Single PCR	48–49	Ward et al. [107]
ldh-A	LDHA6F2	GYGGAGAGCATCSWKAAGAACMTGC	Single PCR	48–49	Quattro & Jones [108]
	LDHA6R*	GCTSAGGAASACCTCRTCCTTCAC	Single PCR	48–49	Quattro & Jones [108]
RPS7	1F	TGGCCTCTTCCTTGGCCGTC	1st PCR	52	Chow & Takeyama [109]
	3R	GCCTTCAGGTCAGAGTTCAT	1st PCR	52	Chow & Takeyama [109]
	1F.2	CTCTTCCTTGGCCGTCGTTG	2nd PCR–1	52	Unmack et al. [74]
	2R.67	TACCTGGGARATTCCAGACTC	2nd PCR–1	52	Unmack et al. [74]
	2F.2.cat	GCCATGTTCAGTACCAGTGC	2nd PCR–2	52	Unmack et al. [74]
	3R.10	TCAGAGTTCATCTCCAGCTC	2nd PCR–2	52	Unmack et al. [74]
X-src	SRC.E7.1F	TGACAGACGTTTGTCCCGTACTGAAGC	1st PCR	52	Peter J. Unmack
	SRC.E10.endR	ATGAGKCGAGCCAGACCGAAATCAGC	1st PCR	52	Peter J. Unmack
	SRC.E8.1F	CTGAAGCCTGGCACCATGTC	2nd PCR	52	Peter J. Unmack
	SRC.E10.end2R	CCGAAATCAGCCACTTTACAMACCAG	2nd PCR	52	Peter J. Unmack
X-yes	Yes F1	GAGAGAATGAACTACATCCATAG	1st PCR	52	Peter J. Unmack
	Yes R1	GACCACACGTCTGATTTGATTGTGAA	1st PCR	52	Peter J. Unmack
	Yes F2	GACAACCTGGTCTGTAAGATCGC	2nd PCR	52	Peter J. Unmack
	Yes R2	GATTTGATTGTGAAGCGACCGTACA	2nd PCR	52	Peter J. Unmack
Glyt	Glyt_F559	GGACTGTCMAAGATGACCACMT	1st PCR	55	Li et al. [110]
	Glyt_R1562	CCCAAGAGGTTCTTGTTRAAGAT	1st PCR	55	Li et al. [110]
	Glyt_F577	ACATGGTACCAGTATGGCTTTGT	2nd PCR	62	Li et al. [110]
	Glyt_R1464	GTAAGGCATATASGTGTTCTCTCC	2nd PCR	62	Li et al. [110]

^§Single PCR, only one PCR performed; 1^st PCR or 2^nd PCR, indicates the sequence in a nested set. Note also that a number n preceded by a dash in this column (e.g. “–2”) indicates the nth second PCR step in a set of nested reactions.