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. 2015 Apr 7;10(4):e0122585. doi: 10.1371/journal.pone.0122585

Fig 3. A decrease in SUMO-conjugated proteins accompanies Shigella infection.

Fig 3

A comparison of SUMO-conjugated proteins from HeLa cells infected with wild type Shigella (M90T), a non-invasive strain (BS176), or uninfected cells for 1, 2, and 3 hours, both strains contained a plasmid expressing the afaE adhesin. Whole cell lysates were prepared from infected cells, separated on 7% polyacrylamide gels and immunoblotted using antibodies specific for SUMO1 (A) or SUMO2/3 (B). In (C) whole cell lysates from HeLa cells infected with wild type Shigella (M90T), a non-invasive mutant defective in the T3SS (mxiD), or an invasive mutant that does not produce a number of effectors (mxiE), was analyzed by immunoblotting using SUMO1-specific antibodies. Immunoblotting using GAPDH served as a loading control.