Figure 3.

Lactobacillus acidophilus NCK2187 and its SlpA protect against pathogenic T-cell-induced colitis

• A B6 Rag1^−/− mice were injected with 10⁶ CD4⁺CD45RB^hi T cells and then orally gavaged with NCK56 (red), NCK2187 (green), or SlpA (blue), 1 and 3 days after transfer, and subsequently once a week for four consecutive weeks, or left untreated (magenta). A group of mice was co-transferred with CD4⁺CD25⁺ T cells as a positive control for protection (Tregs; gray). Colitis severity was determined in part by weight loss, diarrhea scores, and FOB. See Supplementary Tables S1, S2 and S3 for statistical analyses results.
• B, C Colitis scores based on histopathology and gross morphology of the colons of mice treated as in (A) were also used as measures of disease. Scale bar = 50 μm.
• D Circulating levels of proinflammatory cytokines were measured in the sera of the mice transferred and treated as in (A), or sham adoptive transferred (white bars).

Data information: Data represent three individual experiments and are shown as mean ± SEM. n = 5 mice/group. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Black asterisks compare NCK2187 to PBS-treated adoptively transferred mice, and red asterisks to NCK56-treated mice.