Signr3+/+ (WT) or
Signr3−/− (KO) mice were orally gavaged with NCK56, NCK2187, or SlpA on days −1 and −3, and 3% DSS was given in the drinking water. Mice were gavaged with bacteria or purified SlpA every other day for an additional three times, and immunity was analyzed by flow cytometry at day 10.
A Representative plots indicate the frequency of neutrophils in the colons of untreated or DSS-treated WT (left) and KO mice (right). Empty bars = WT; lined bars = KO; white bars = untreated; purple bars = DSS; red bars = DSS + NCK56; green bars = DSS + NCK2187; blue bars = DSS + SlpA.
B, C Colonic DCs and macrophages (MΦs) were analyzed by flow cytometry for the production of IL-1β (B), and colonic FoxP3+ Tregs were evaluated for co-expression of RORγt+ (C). n = 5 mice/group. Gray tinted line = isotype control; black = untreated; purple = DSS; red = DSS + NCK56; green = DSS + NCK2187; blue = DSS + SlpA.
Data information: Data are shown as mean ± SEM and are representative of three different experiments. *
P < 0.05, **
P < 0.01, ***
P < 0.001, ****
P < 0.0001.