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. 2015 Feb 23;34(7):940–954. doi: 10.15252/embj.201489642

Figure 3.

Figure 3

The apical α-βH Spectrin cytoskeleton binds to and co-localises with Expanded protein and acts genetically upstream or at the level of Expanded to control wing growth
  • A Co-IP of V5-tagged Expanded with the FLAG-tagged N-terminal region of Karst/βH-Spectrin, but not other Karst truncation constructs.
  • B, C Expanded co-localises with Karst at the apical membrane of wing imaginal disc cells (apical view, B; cross section, C).
  • D Control nubbin.Gal4 expressing wing.
  • E Overexpression of Ex activates Hippo signalling to produce a small wing.
  • F RNAi knock-down of α-Spectrin results in an overgrown wing.
  • G Overexpression of Ex blocks the effect of α-Spectrin RNAi.
  • H RNAi knock-down of βH-spectrin/karst results in an overgrown wing.
  • I Overexpression of Ex blocks the effect of βH-spectrin/karst RNAi.
Data information: Scale bars, 10 μm (B, C), 250 μm (D–I).