Figure 7.
Basolateral α-β Spectrins are required to restrict cell proliferation in the Drosophila intestinal epithelium
- A Control myo1A.Gal4 UAS.GFP adult midgut stained for phospho-histone H3 to mark mitotic stem cells.
- B-E RNAi knock-down of βH-spectrin/karst (B) or crumbs (C) does not increase stem cell proliferation, while RNAi knock-down of β-spectrin (D) or α-spectrin (E) strongly stimulates stem cell proliferation.
- F Overexpression of Yki strongly stimulates stem cell proliferation.
- G Quantification of the number of PH3-positive mitotic cells. N > 13 samples. Statistical analysis performed using the two-tailed Student's t-test. Error bars show standard deviation. ***P-value ≤ 0.001.
- H-K A Hippo reporter DIAP1-HRE-GFP is normally expressed in sporadic stem cells. Its expression is not affected by RNAi knock-down of βH-spectrin/karst (I), but is up-regulated in many stem cells by RNAi knock-down of α-spectrin (J) or by overexpression of Yki (K).