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. 2015 Feb 23;34(7):940–954. doi: 10.15252/embj.201489642

Figure 8.

Figure 8

Human Spectrin SPTAN1 and SPTBN1 are required for the YAP localisation response to cell density
  • A Control Caco-2 epithelial cells plated a low density show strong nuclear YAP localisation.
  • B Control Caco-2 epithelial cells plated at high density show relocalisation of YAP to the cytoplasm.
  • C, D siRNA knock-down of SPTAN1 (C) or SPTBN1 (D) prevents relocalisation of YAP to the cytoplasm.
  • E Quantification of YAP localisation in (B–D).
  • F Western blotting reveals that SPTAN1 or SPTBN1 are required for normal levels of YAP phosphorylation, and of LATS1 phosphorylation.
  • G siRNAs against SPTAN1 or SPTBN1 efficiently deplete their target proteins.
Data information: Scale bars, 20 μm.