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. 2015 Feb 23;16(4):481–489. doi: 10.15252/embr.201440015

Figure 2.

Figure 2

Annexin A2 downregulation abrogates the actomyosin ring formation
  • A Spinning confocal images of HeLa cells expressing the filamentous actin biosensor Life Act. Anx2 siRNA-transfected cells undergoing asymmetric furrow ingression display decreased actin accumulation on the non-invaginating side of the equatorial cortex (arrow) compared to the Ctl RNAi-treated cells. For full sequence, see Supplementary Movies S6 and S7.
  • B-D HeLa cells treated with Ctl or Anx2 siRNA were synchronized at G2–M transition, released to reach anaphase and early telophase and then analyzed by immunofluorescence for contractile ring elements. Cells were analyzed for F-actin with phalloidin (B) and antibody against myosin II (C). Percentage of cells displaying normal equatorial actin staining following transfection with Ctl or Anx2 siRNA (B, graph). Error bars, SD of three experiments (n ≥ 100). *P = 0.001, Student's t-test. Cells were analyzed for anillin (D). Percentages indicate the fraction of cells displaying the phenotype represented in the image among the cells scored in anaphase (n = 80). Quantification of the percentage of cells presenting normal equatorial anillin staining (D, graph). Error bars, SD of three experiments (n ≥ 60 each). *P = 0.001, Student's t-test. Scale bars, 10 μm.