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. 2015 Feb 5;16(4):490–499. doi: 10.15252/embr.201439410

Figure 2.

Figure 2

Increased microtubule assembly rates in response to loss of Wnt signaling cause abnormal mitotic spindles and lagging chromosomes during anaphase
  1. Mitotic microtubule plus end assembly rates in different single cell clones derived from HCT116 cells stably expressing shRNAs targeting LRP6 or DVL2. Scatter dot plots show average assembly rates (20 microtubules/cell, mean ± SEM, t-test, n = 20 cells).
  2. Quantification of cells showing abnormal bipolar metaphase spindles. Cells were synchronized in metaphase as indicated. The graph shows the induction of abnormal spindles associated with incomplete chromosome alignment normalized to control cells (mean ± SD, n = 800–1,000 mitotic cells). Representative examples are shown (scale bar, 10 μm).
  3. Detection of lagging chromosomes in HCT116 cells transfected with siRNAs and synchronized in anaphase. Cells were treated with or without 0.5 nM Taxol®, and the proportion of cells exhibiting lagging chromosomes were quantified (mean ± SD, n = 371–711 anaphase cells). Representative examples are shown (scale bar, 10 μm).