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. 2015 Feb 5;16(4):490–499. doi: 10.15252/embr.201439410

Figure 3.

Figure 3

Loss of Wnt signaling induces aneuploidy mediated by increased mitotic microtubule assembly rates
  1. Chromosome number variability/aneuploidy of different single cell clones derived from HCT116 cells stably expressing control or shRNAs targeting LRP6 and DVL2 and grown for 30 generations. The graph shows the proportion of cells harboring a karyotype with chromosome numbers deviating from the modal (n = 50 cells; modal number = 45). Representative examples of metaphase chromosome spreads showing a normal (45,X) and an aneuploid (44,X) karyotype are shown.
  2. Mitotic microtubule plus end assembly rates in different single cell clones derived from HCT116 cells stably expressing shRNAs targeting LRP6 or DVL2 and grown for 30 generations in the absence (DMSO) or presence of low doses of Taxol®. Scatter dot plots show average assembly rates (20 microtubules/cell, mean ± SEM, t-test, n = 10 cells).
  3. Determination of the chromosome number variability/aneuploidy in different single cell clones grown in the absence or presence of Taxol® for 30 generations. The graph shows the proportion of cells harboring a karyotype with chromosome numbers deviating from the modal (n = 50 cells; modal number = 45).