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. 2015 Feb 9;16(4):528–538. doi: 10.15252/embr.201439092

Figure 5.

Figure 5

Knockdown of H3.3 restores dendrite and arista development in dBRWD3 knockdown flies
  • A-D Dendrite morphogenesis regulated reciprocally by dBRWD3 and H3.3. Dendrite morphogenesis of class IV da neuron was highlighted by UAS-mCD8-GFP driven by ppk-GAL4. (A) Specific knockdown of dBRWD3 in class IV da neurons achieved by ppk-GAL4-driven UAS-dBRWD3-dsRNA, UAS-mCD8-RFP resulted in the absence of high-order dendritic branches (arrow). (B) High-order dendritic branches (arrow) increased in ppk-GAL4-driven UAS-mCD8-GFP control. (C) High-order dendritic branches (arrow) were restored in animals that simultaneously express UAS-dBRWD3-dsRNA and UAS-H3.3B-dsRNA by ppk-GAL4. (D) The mean dendritic branch number at 225 μm away from the soma from 27 independent experiments was shown. The error bars indicate 1 SD. *Indicates P < 0.00001 versus GAL4 control and **indicates P < 0.00001 versus dBRWD3 knockdown by Student's t-test. Scale bars indicate 100 μm.
  • E-H Arista development is regulated by dBRWD3 and H3.3. (E) Knockdown of dBRWD3 in developing arista was achieved by dpp-GAL4-driven UAS-dBRWD3-dsRNA, UAS-mCD8-RFP. The number of branches and length were reduced in the dBRWD3-depleted arista. (F) Normal arista consists of 8–9 branches. (G) Both number of branches and length were restored in the dBRWD3, H3.3B doubly depleted arista. (H) The number of arista branches from 28 independent experiments was shown. The error bars indicate 1 SD. *indicates P < 0.00001 versus GAL4 control and **indicates P < 0.00001 versus dBRWD3 knockdown by Mann–Whitney U-test. Scale bars indicate 100 μm.