Fig 2. The mRNA expression of p63 gene variants is dependent on seeding cell density of hMSC.
hMSC were seeded at various densities, ranging from 500–30,000 cell/cm2, and then grown under expansion conditions for 3-days followed by RT-qPCR analysis of p63 gene products. RT-qPCR analysis of p63 isoforms (left panel, TA- and ΔNp63) and splice variant mRNA (right panel, p63α / β and p63γ) expression. N = 3 independent experiments in triplicate. (*) p ≤ 0.05 compared to hMSC seeded at 500 cells/cm2, which were set to the value of “1”. hMSC used were from a 7 and 22 year old male.