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. 2015 Apr 7;10(4):e0120601. doi: 10.1371/journal.pone.0120601

Fig 3. Expression profile of recombinant wild-type and mutant forms of Lcc4 in the cultural media of P. pastoris X33 cells.

Fig 3

(A) Time course of yeast host cell growth and protein expression and concentration. (B) CBR stained gel and zymogram of cultural media. For zymogram analysis, the snapshots of SDS gel were immersed in 2 mM ABTS solution for 20 and 60 s, respectively. Protein marker (M); vector (lane 1); wild-type (lane 2); N75D (lane 3); N162D (lane 4); N238D (lane 5); N458D (lane 6). (C) Relative laccase specific activity in the cultural medium of yeast cells transformed with vector only, wt, N75D, N162D, N238D, and N48D gene construct.