Table 1. Nucleotide sequences of PCR primers.
Primer | Oligonucleotide sequence |
---|---|
lcc4F | 5′-TTTTATGAAGGCCCTCTCCTTCCTTACACC-3′ |
lcc4R | 5′-TTTTTCACTGGTCGTCCTCAGCTAGG-3′ |
lcc4F_no sp | 5′-TTTGAATTCACTCCGTCGGGCCCGTAGCCAACCTCAAGATCG-3′ |
lcc4R_no sp | 5′-TTTGCGGCCGCTCACTGGTCGTCCTCAGCTAGGGCGTCGTAG-3′ |
N75D_F | 5′-CGATGAGCTGACGGACCACACTATGCTC-3′ |
N75D_R | 5′-GAGCATAGTGTGGTCCGTCAGCTCATCG-3′ |
N162D_F | 5′-GTATGATGTTGATGACGAATCTACGGTGATC-3′ |
N162D_R | 5′-GATCACCGTAGATTCGTCATCAACATCATAC-3′ |
N238D_F | 5′-CAGCATCGATGGTCACGATATGACCATTATC-3′ |
N238D_R | 5′-GATAATGGTCATATCGTGACCATCGATGCTG-3′ |
N458D_F | 5′-GCGGCCGGCGACGACGTGACGATCCGTT-3′ |
N458D_R | 5′-AACGGATCGTCACGTCGTCGCCGGCCGC-3′ |