Table 3. Kinetic properties of native (nLcc4) and Endo H-deglycosylated lcc4 (dLcc4) ^a .

Protein	Substrate	Specific activity (U/mg) b	k cat (s −1)	K m (μM)	k cat/K m (s −1μM−1)	Optimal reaction conditions (°C/pH)
nLcc4	ABTS	2996	3382	65.0±6.5	52	70/2.5
dLcc4	ABTS	2342	2488	48.3±5.5	52	70/2.5
nLcc4	2,6-DMP	119	134	412.1±28.0	0.33	60/3.5
dLcc4	2,6-DMP	114	121	437.1±23.9	0.28	60/3.5

^a The enzymatic reaction was performed at the respective optimal temperatures and pHs as indicated.

^b One unit of enzyme activity was defined as the amount of enzyme that oxidizes 1 μmol of substrate per minute under optimal reaction conditions.