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. 2015 Apr 7;10(4):e0122786. doi: 10.1371/journal.pone.0122786

Fig 1. Cell viability curves for Diclofenac, Chlorpromazine, Acetaminophen, and Verapamil.

Fig 1

5000 human primary hepatocytes pooled from either 12 male donors (M), or 12 pre-menopausal female donors (3F), or 12 post-menopausal female donors (4F) were seeded in 96 well plates and exposed to 8 serial concentrations (using a dilution factor of 1:2) of Diclofenac (from 13.6 to 1750 uM), Chlorpromazine (from 1.9 to 250 uM), Acetaminophen (from 0.3 to 35 uM), or Verapamil (from 7.8 to 1000 uM). Decrease in cell number after 30 min, 2h, or 5h exposure to drugs was assessed by lysing the cells with CellTiter-Glo Reagent and measuring the ATP levels released. Assays were run in triplicates. Results are expressed as mean ±SEM. Dose response curves were plotted using GraphPad Prism.