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. 2015 Apr 7;10(4):e0122786. doi: 10.1371/journal.pone.0122786

Fig 2. Cell viability curves for Omeprazole and Caffeine.

Fig 2

5000 human primary hepatocytes pooled from either 12 male donors (M), or 12 pre-menopausal female donors (3F), or 12 post-menopausal female donors (4F) were seeded in 96 well plates and exposed to 8 serial concentrations (using a dilution factor of 1:2) of Omeprazole (from 1.9 to 250 uM), or Caffeine (from 39 to 5000 uM). Cell viability after 30 min, 2h, or 5h exposure to drugs was assessed by lysing the cells with CellTiter-Glo Reagent and measuring the ATP levels released. Assays were run in triplicates. Results are expressed as mean ±SEM. Data were plotted using GraphPad Prism.