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. 2015 Apr 7;10(4):e0123270. doi: 10.1371/journal.pone.0123270

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© 2015 Kim et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — (A) Amino acid sequence alignment between NppDI and NppY glycosyltransferases in NPP biosynthesis. Regions for mutagenesis are marked; crosshatch, site-directed mutagenesis (dark gray); asterisk, addition of 7 consecutive amino acids (FARSYTP) from NppDI (light gray); arrow, N-terminal of NppY and C-terminal of NppDI were combined based on straight line. (B) NPP conversion rate through expression of various modified nppY genes in ESK6011.