Table 1. Bacterial strains and plasmids used in this study.

Strain of plasmid	Characteristic a	Source or reference
Strain
Escherichia coli
DH5α	General cloning host
ET12567 / pUZ8002	Strain for intergeneric conjugation; Kmr, Cmr	[37]
Pseudonocardia autotrophica
KCTC9441	Wild-type, NPP producing strain	KCTC
ESK601	nppDI mutant producing 10-deoxyaglycone	[23]
ESK602	nppDI-complemented ESK601	[23]
ESK6011	nppY mutant producing 10-deoxynystatin	This work
ESK6012	nppY-complemented ESK6011	This work
ESK60111	ESK6011 harboring plasmid pYM1	This work
ESK60112	ESK6011harboring plasmid pYM2	This work
ESK60113	ESK6011 harboring plasmid pYM3	This work
ESK60114	ESK6011 harboring plasmid pYM4	This work
ESK60115	ESK6011 harboring plasmid pYM5	This work
ESK60116	ESK6011 harboring plasmid pYM6	This work
ESK60117	ESK6011 harboring plasmid pYM7	This work
ESK60118	ESK6011 harboring plasmid pYM8	This work
ESK60119	ESK6011 harboring plasmid pYM9	This work
ESK6021	nppL mutant producing C10 deoxy NPP	This work
ESK6022	nppL-complemented ESK6021	This work
ESK611	nppDI nppY double mutant producing 10-deoxyaglycone	This work
ESK612	nppDI-complemented ESK611	This work
ESK613	nppY-complemented ESK611	This work
ESK614	nppDI nppY-double complemented ESK611	This work
ESK621	nppDI nppL double mutant producing 10-deoxyaglycone	This work
ESK622	nppDI-complemented ESK621	This work
ESK623	nppL-complemented ESK621	This work
ESK624	nppDI nppY-double complemented ESK621	This work
ESK631	nppY nppL double mutant producing 10-deoxynystatin	This work
ESK632	nppY-complemented ESK631	This work
ESK633	nppL-complemented ESK631	This work
ESK634	nppY nppL-double complemented ESK631	This work
ESK641	nppDI nppY nppL triple mutant producing 10-deoxyaglycone	This work
ESK642	nppDI-complemented ESK641	This work
ESK643	nppDI nppY-complemented ESK641	This work
ESK644	nppDI nppY nppL-complemented ESK641	This work
Plasmid
pTY	Template plasmid for nppY mutation, Amr	This work
pDELY	Plasmid for deletion nppY gene, Aprr	This work
pDELL	Plasmid for deletion nppL gene, Aprr	This work
ermE*pSET152	E. coli-P. autotrophica conjagative vector with constitutive promoter, ermE*, Aprr	[38]
pMJPDI	Plasmid for nppDI complementation, Aprr	[23]
pPY	Plasmid for nppY complementation, Aprr	This work
pPL	Plasmid for nppL complementation, Aprr	This work
pPDIY	Plasmid for nppDI nppY complementation, Aprr	This work
pPDIL	Plasmid for nppDI nppL complementation, Aprr	This work
pPYL	Plasmid for nppY nppL complementation, Aprr	This work
pYM1	pPY with additional 7 amino acids (FARSYTP), Hygr	This work
pYM2	pPY with A37N mutation, Hygr	This work
pYM3	pPY with E197K mutation, Hygr	This work
pYM4	pPY with R200N mutation, Hygr	This work
pYM5	pPY with I201Q mutation, Hygr	This work
pYM6	pPY with R219V mutation, Hygr	This work
pYM7	pPY with R247E mutation, Hygr	This work
pYM8	pPY with I385Y mutation, Hygr	This work
pYM9	pPY combined with C-terminal domain of NppDI, Hygr	This work

^aKm^r, kanamycin resistance; Cm^r, chloramphenicol resistance; Am^r, ampicillin resistance; Apr^r, apramycin resistance; Hyg^r, hygromycin resistance.