Table 2. Generated P. autotrophica mutant strains and compounds through both gene deletion and complementation.
| Deletion | Complementation | ||
|---|---|---|---|
| Mutant strain | Resulted compound | Complementing gene | Resulted compound |
| ESK601(ΔnppDI) | 10-deoxyaglycone | nppDI(ESK602) | NPP |
| ESK6011(ΔnppY) | 10-deoxynystatin | nppY(ESK6012) | NPP |
| ESK6021(ΔnppL) | 10-deoxyNPP | nppL(ESK6022) | NPP |
| ESK611(ΔnppDIΔnppY) | 10-deoxyaglycone | nppDI(ESK612) | 10-deoxynystatin |
| nppY(ESK613) | 10-deoxyaglycone | ||
| nppDI&nppY(ESK614) | NPP | ||
| ESK621(ΔnppDIΔnppL) | 10-deoxyaglycone | nppDI(ESK622) | 10-deoxyNPP |
| nppL(ESK623) | 10-deoxyaglycone | ||
| nppDI&nppL(ESK624) | NPP | ||
| ESK631(ΔnppYΔnppL) | 10-deoxynystatin | nppY(ESK632) | 10-deoxyNPP |
| nppL(ESK633) | 10-deoxynystatin | ||
| nppY&nppL(ESK634) | NPP | ||
| ESK641(ΔnppDIΔnppYΔnppL) | 10-deoxyaglycone | nppDI(ESK642) | 10-deoxynystatin |
| nppDI&nppY(ESK643) | 10-deoxyNPP | ||
| nppDI&nppY&nppL(ESK644) | NPP | ||