Fig 5. eIF4G is required in the somatic cyst cells for normal spermatogenesis.

Wild-type testes (A-C') and testes expressing eIF4G shRNA with c587-Gal4 driver (A''-C''') were co-stained with anti-eIF4G (red) and anti-eIF4E-3 (green) antibodies. Loss of anti-eIF4G staining in the cyst cells at the apical tip of testis (B'') indicates efficient knockdown of the protein. The testis shows an increased number of abnormal germ cell cysts at its apical tip (B'') and reduced meiotic and post-meiotic cysts along its length (compare A' & C' with A''' & C''') as revealed by anti-eIF4E-3 staining (green). Although mature sperm nuclear bundles are found at the distal end of the testes, they appear disorganised (C''', arrows) and nuclei at various stages of condensation are also found at the caudal end of the elongating spermatids (B''', arrows).