. 2015 Apr 7;10(4):e0123668. doi: 10.1371/journal.pone.0123668

Table 1. Oligonucleotide sequences for primers used in this study.

Gene name	Gene bank accession number	Prime sequences (5’–3’)	Purpose
DkXTH1	JN605344	F: `ACGCCAAGTTCTGCGACAC`	DkXTH1 RT-qPCR
		R: `GGGTATCGCTTCCTGTCG`
		F: `CGGGATCCAAAGGCGGCAACTTCTACC`	DkXTH1 recombinant protein expression
		R:`CCCAAGCTTCTAGAGGAACCTCGACCT`
		F:`GCTCTAGAATGGCATTCATGTCCTTTC`	DkXTH1Full
		R:`GGGGTACCGAGGAACCTCGACCTCC`
		F:`GCTCTAGAATGGCATTCATGTCCTTTC`	DkXTH1sp
		R:`GGGGTACCGGCGGCCATCATAGAGCT`
		F:`GCTCTAGAAAAGGCGGCAACTTCTA`	DkXTH1Int
		R:`GGGGTACCGAGGAACCTCGACCTCC`
DkXTH2	JN605345	F:`ACGCCAAGTTCTGCGACAC`	DkXTH2 RT-qPCR
		R: `GGGTATCGCTTCCTGTCG`
		F:`CGGGATCCGTACCCAGGAAGCCCGTT`	DkXTH2 recombinant protein expression
		R:`CCGCTCGAGTTATATGTCTCTGTCTCTTTTGC`
		F:`GCTCTAGAATGGCGATGGGTACCCACTT`	DkXTH2Full
		R:`CCGCTCGAGTATGTCTCTGTCTCTTTTGCACTCG`
DkXTH3	JN605346	Outer: `CCTTGTAGTAGGCGTAGAATGG`	DkXTH3 5’RACE
		Inner: `CCCGCTCCTATTTCCCAAGA`
		Outer: `CGCATGGGAAGGGTGATAGG`	DkXTH3 3’RACE
		F:`ACATTGGCATGGAAGAAC`	DkXTH3 full-length cDNA clone
		R:`CCCCTCGACTGCCAAACA`
		F:`GGTGATAGGGAGCAAAGG`	DkXTH3 RT-qPCR
		R:`GGACCTTCGGGTATGGA`
DkXTH4	JN605347	Outer: `GAAGGAAGAAGAGCTGGAAGT`	DkXTH4 5’RACE
		Inner: `AGGTTGCTGTGAAGGGTG`
		Outer: `TCACCGGAGGAAAGGGCGAC`	DkXTH4 3’RACE
		F:`ACAAACCCATCAACCAACT`	DkXTH4 Full-length cDNA clone
		R:`AACTTCAGGGCAAACAGAG`
		F:`ACTTCAATGCCCAGACC`	DkXTH4 RT-qPCR
		R:`ACAGCCATTTCTTTAGGG`
DkXTH5	KC511052	Outer: `TCCGCTTTGAAGTTCCTGTA`	DkXTH5 5’RACE
		Inner: `AAGTCTATCTCGTCGTGGGT`
		Outer: `TGGACGGCACGCCCATCAGA`	DkXTH5 3’RACE
		F:`CATTGTATCCAGAAGGCAGAGC`	DkXTH5 full-length cDNA clone
		R:`CAGCGGATTGCGCTTGAT`
		F:`CCGCTGACCTCAACCAA`	DkXTH5 RT-qPCR
		R:`TCCCTGCGACGACAGATAG`
Actin	AB219402	F:`TGCTCTTCCAGCCATCACTCATT`	Actin RT-qPCR
Actin		R:`ATTTCCTTGCTCATCCGGTCAG`

Letters “F” and “R” indicate the forward and reverse primers, respectively.