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. 2015 Apr 7;10(4):e0123325. doi: 10.1371/journal.pone.0123325

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Fig 2 — Upper panels: 24-hour collection (A-J). Tissue localization of Bmp2CreER activity at 24 hours after tamoxifen (Tam) treatment on consecutive days from E10.5–14.5. Panels A,C,E,G,I (forelimb, FL) and B,D,F,H,J (hindlimb, HL) show longitudinal sections through limb dorsoventral axis oriented with distal at top, and anterior-to-posterior (digit 1–5) from left-to-right. Hatch marks in panels C-J show the levels at which cross-sections below panels C-J were taken (distal to proximal). Cross-sections are all oriented with dorsal at top and anterior-to-posterior (digit 1–5) from left-to-right. Lower panels: E13.5 collection (K-N). Tissue distribution of LacZ activity at E13.5 after tamoxifen (Tam) treatment at E10.5 (K,L) or E11.5 (M,N). For each treatment time, whole mount and adjacent longitudinal and cross-sections of forelimb (FL) or hindlimb (HL) are shown. Hatch marks in panels of longitudinal sections show the levels at which cross-sections through digit region were taken (distal to proximal). Longitudinal and cross-sections are oriented as for Upper Panels. Whole mount images are oriented the same as longitudinal sections.