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. Author manuscript; available in PMC: 2015 Apr 7.
Published in final edited form as: Small. 2007 Sep;3(9):1549–1559. doi: 10.1002/smll.200700200

Figure 2.

Figure 2

A) SDS-PAGE analysis (8% gel) after Coomassie staining and B) Western-blot analysis using an antiRmlA antibody of biocatalytic proteins. Lane 1: Bench-mark ladder (Invitrogen); lane 2: purified C_SgsE (control); lane 3: purified G_SgsE (control); lane 4: raw extract of a C_SgsE.RmlA expression culture; lane 5: purified C_SgsE.RmlA; lane 6: raw extract of a G_SgsE.RmlA expression culture; lane 7: purified G_SgsE.RmlA; lane 8: purified C_SgsE.RmlA after Western blotting; lane 9: purified G_SgsE.RmlA after Western blotting. 20 μg (raw extracts) and 10 μg (purified proteins) of total protein were loaded on the gel for Coomassie staining; ≈2 μg were loaded for Western blotting.