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. Author manuscript; available in PMC: 2015 Apr 7.
Published in final edited form as: Small. 2007 Sep;3(9):1549–1559. doi: 10.1002/smll.200700200

Table 1.

Oligonucleotide primers used for PCR amplification of different forms of sgsE and of rmlA.

Primer Nucleotide sequence (5′→3′)
A_sgsE for(NcoI) AATCACC Inline graphic [a]gCGGACGTGGCGACGGTCG
C_sgsE for(NcoI) AATCACC Inline graphic gCAAAATTAGACAAAATGCGCC
G_sgsE for(NcoI) AATCACC Inline graphic gTAAAATTAGTGGTTGATGGCGC
sgsE_rev(XhoI;+Stop) AATCA Inline graphic CTCGAGTTTTGCTACGTTTACAACAGTAGC
sgsE_rev(XhoI;−Stop) AATCACTCGAGTTTTGCTACGTTTACAACAGTAGC
sgsE_rev(KpnI,XhoI;+Stop) AATCA Inline graphic CTCGAGGGTACCTTTGCTACGTTTACAACAGTAGC
C-term. rmlA_for(KpnI) AATCAGGTAccgcAAGGTATCGTTCTAGCCGG
C-term. rmlA_rev(XhoI) AATCACTCGAGGAAAGGCATTACTAAACTTAGTCTCAAT
rmlA_for (NdeI) AATCACAT Inline graphic gAAGGTATCGTTCTAGCCGGCG
[a]

Artificial restriction sites are underlined; lowercase letters indicate changes in the chromosomal DNA sequence to introduce appropriate restriction sites. The triplets corresponding to the initiation and termination codons in the primer sequence are boxed.