Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Apr 7;10(4):e0122365. doi: 10.1371/journal.pone.0122365

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Mazzone et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Fig 2 — A) Immunoblot analysis of lysates from HEK293 cells transfected with and expression vector empty (vector) or expressing HA-zBcl10 and HA-hBCL10. 24 hrs after transfection, cell lysates were prepared, separated by SDS/PAGE, and analyzed by immunoblot assay probed with anti-HA (left panel) and anti-BCL10 (right panel) antisera. An arrow indicates hBCL10 endogenously expressed by HEK293 cells. B) Cell lysates from HEK293 transfected cells were prepared as in A). Were indicated, before SDS/PAGE separation cell lysates were treated with 10 units of calf intestinal phosphatase (CIP) for 30 min at 37°C. C) The same experiment shown in B) was carried out in the embryonic zebrafish fibroblast cell line PAC2. D) Immunoblot analysis of proteic extracts from zebrafish organs probed with an anti-hBCL10 antisera. Cell extract from HEK293 cells transfected with zBcl10 was used as a positive control (arrow).