Skip to main content
. 2015 Apr 7;10(4):e0122365. doi: 10.1371/journal.pone.0122365

Fig 5. zBcl10 replaces hBCL10.

Fig 5

A) HEK293 cells were infected with recombinant retrovirus expressing shRNAs targeting hBCL10. 72 hrs later, hBCL10 expression was monitored by immunoblot assay. B) NF-κB-driven luciferase activity in HEK293 cells silenced for hBCL10 and stimulated with PMA. C) NF-κB-driven luciferase activity in HEK293 cells silenced for hBCL10 and transfected with zBcl10. Lower panel: A fraction of the cell lysates were analyzed by an immunoblot assay probed with anti hBCL10 to monitor protein expression. Data shown (mean + SEM, n = 9) represent relative luciferase activity normalized on β-galactosidase activity and is representative of six independent experiments done in triplicate. Statistical analysis was performed by Student's t test; a p value of <0.05 was considered significant, and is indicated with the symbol *.