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. 2015 Apr 7;10(4):e0121887. doi: 10.1371/journal.pone.0121887

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© 2015 Li et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 1 — A. pleuropneumoniae was cultured in TSB medium overnight and then sub-cultured into CDM at a dose of 10⁴ CFU/ml. Optical densities of bacterial cultures (OD_600nm) were recorded at selected time points. (A) Various concentrations of serum (0–1/10; V/V) were added into CDM; (B) Serum at the concentration of 1/40 was added into CDM (CDM + S). FeCl₃, MgCl₂ and ZnCl₂ were supplemented into the serum-containing medium respectively (CDM + S + Fe/Mg/Zn). A. pleuropneumoniae cultured in CDM without any supplementation was used as a control (CDM). Data are shown as means ± SD from four independent replications.