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. 2015 Apr 7;10(4):e0121887. doi: 10.1371/journal.pone.0121887

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© 2015 Li et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — A. pleuropneumoniae was cultured in TSB medium overnight and then sub-cultured into CDM using an inoculation dose of 10⁴ CFU/ml. ATF at 400μg/ml was added into CDM (ATF). Epi, NE and DA at 50μM were supplemented into the medium containing ATF (ATF + Epi/NE/DA). A. pleuropneumoniae cultured in CDM without any supplementation was used as a control (CDM). (A). Optical densities of bacterial cultures (OD_600nm) were recorded at selected time points. (B). Bacterial viable numbers were recorded at 6 h, 8h and 10h. Data are shown as means ± SD from four independent replications.