Table 1. The optimized cone voltages (CV) and collision energies (CE) for protonated ions of vasicine and its main metabolites.

Metabolites	Precursor ions (m/z)	CV (V)	CE (V)
M0	189.1028	30	10–30
M1	203.0821	25	10–30
M2	203.1184	30	20–30
M3	205.0977	25	10–30
M4	217.0613	30	10–20
M5	219.0770	30	10–30
M6	219.1134	25	10–20
M7	221.0926	30	10–20
M8	235.0719	35	10–20
M9	235.1083	25	10–20
M10	237.0875	30	20–30
M11	245.1290	30	10–30
M12	247.1083	25	20–30
M13	251.1032	30	10–30
M14	261.1239	30	10–20
M15	269.0596	25	20–30
M16	283.0389	25	20–30
M17	285.0545	30	10–20
M18	297.0181	30	10–20
M19	299.0338	35	20–30
M20	365.1349	30	10–20
M21	379.1141	25	20–30
M22	381.1298	20	10–30
M23	393.0934	30	20–30
M24	395.1091	25	10–20
M25	397.1247	30	10–30
M26	411.1404	35	10–20
M27	413.1196	25	10–20
M28	421.1611	25	10–20
M29	437.1560	25	10–20
M30	557.1619	30	10–30