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. Author manuscript; available in PMC: 2016 May 15.
Published in final edited form as: Vet Microbiol. 2015 Feb 16;177(0):7–17. doi: 10.1016/j.vetmic.2015.02.008

Table 2.

Survival, body temperatures, body weights and HI titers of ducks inoculated with vNDV, LPAIV and HPAIV, given single or combined.

Groups Day of inoculationa
# dead/ total (MDT)b Body temperaturec 2 dpi Body weightsc 2 dpi HI titersd
Day 0 Day 2 NDV AIV
1 - - 0/9 107.0±0.6A 694±55AB ND ND
2 vNDV - 0/9 107.5±0.5A 638±81AB 7/7 (5.7+.9) ND
3 LPAIV - 0/9 107.7±0.8A 705±12A ND 7/7 (3.8+.3)
4 HPAIV - 9/9 (2.3)AB 103.6±4.3B 576±27B ND ND
5 vNDV +LPAIV - 0/9 107.6±0.5A 682±49AB 7/7 (5.8+.6) 6/7 (3)
6 vNDV +HPAIV - 9/9 (2.1)A 107.9±2.6A 672±73AB ND ND
7 vNDV LPAIV 0/9 108.0±0.8A 838±79A 7/7 (5.2+.7) 0/7
8 vNDV HPAIV 9/9 (3.2)B 109.5±0.8B 748±113A ND ND
9 LPAIV vNDV 0/9 107.6±0.8A 867±130A 7/7 (5.5+.5) 4/7 (3.2+.5)
10 HPAIV vNDV 9/9 (2.3)AB ND - ND ND
a

Two-week-old Pekin ducks were inoculated with 107 EID50 of vNDV, 107.5 EID50 of LPAIV, and 106 EID50 of HPAIV administered in 0.1 ml split between the right eye and the choana. The viruses were given alone, simultaneously, or sequentially (the second virus 2 days after the first).

b

Mean death time calculated after exposure to the HPAIV. Survival in groups with different uppercase letters is significantly different (P<0.05).

c

Body temperature (F) and weights in groups with different uppercase letters are significantly different (P<0.05).

d

Mean HI titers (log2) in ducks. Serum samples were taken at 8 or 10 days after infection with LPAIV, vNDV, or both. The number of birds with positive HI titers is shown. ≥ threshold of positivity/total number of sera tested (mean ± SEM).

ND = no data