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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Methods. 2015 Feb 16;0:125–135. doi: 10.1016/j.ymeth.2015.02.004

Figure 1.

Figure 1

Principle of stopped-flow fluorescence experiment. Top: The kinetics of PTEN binding is investigated by mixing PTEN with dansyl-PE labeled lipid vesicles. TRP and Dansyl form a FRET pair and the PTEN bilayer association can be followed by monitoring either the decrease of the TRP or the increase of the dansyl fluorescence (shown here). Bottom: The kinetics of PTEN bilayer dissociation is followed by mixing PTEN bound to labeled lipid vesicles with an excess of unlabeled lipid vesicles (except for the labeled lipid, both types of lipid vesicles have the same lipid composition).