Table 4. Steps of PTEN function and corresponding characterization methods in lipid model systems.
Equilibrium | Kinetics | Dynamics | Conformation | |
---|---|---|---|---|
Association | TRP quenching/SPR | Stopped-Flow/SM TIRF | Neutron Diffraction | |
Lateral Distribution | Fluorescence Microscopy (Langmuir Films/GUVs) | SM TIRF | ||
Activation | Interfacial kinetics | IR Spectroscopy | ||
Hydrolysis | Interfacial kinetics | |||
Dissociation | Stopped-Flow SM TIRF |