Fig 3. Effects of SR-A or CD36 deficiency on antigen uptake, expression of endocytic receptors and antigen presentation to CD4⁺ OT-II splenocytes.

(A, D) Uptake of indicated, fluorescently labelled proteins, present at 5 μg/ml by BM-DC (A) and PEM (D) was assessed by flow cytometry. (B, E) Specific binding of Ab to receptors (geometric mean fluorescence intensity) on BM-DC (B) and PEM (E) which was obtained by subtracting binding of control Ab from the total binding of receptor-specific Ab. (C, F) IL-2 production in 1- or 2-days co-cultures of BM-DC (C) or PEM (F) with CD4⁺ OT-II lymphocytes. Directly before the co-incubation with lymphocytes, APC were pulsed for 3.5 h with 20 μg/ml OVA or 7 μg/ml OVA-Cl. The data shown on graphs A-G are means +SEM from 6–8 independent experiments. (H) Titers of OVA- or HSA-specific IgM in sera of mice immunized 8 days earlier with 20 μg OVA-Cl or HSA-Cl. Points represent titer values in individual mice and horizontal lines geometric means. The data were analysed with the regular (H) or repeated measures (A-G) ANOVA and the Dunnett’s post-test was used to make comparisons with the control groups (WT). *, p < 0.05.