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. 2015 Apr 7;11(4):e1005145. doi: 10.1371/journal.pgen.1005145

Fig 1. Proteolysis of ToxR during late stationary phase at alkaline pH.

Fig 1

(A) ToxR immunoblot of O395 wild-type or ΔtoxR grown for either 12 or 48 hours in LB starting pH 7.0 unbuffered (LB), LB starting pH 9.3 unbuffered (pH 9.3), or LB buffered to pH 7.0 with 100 mM HEPES (Buff). (B) ToxR immunoblots of O395 wild-type grown at different time points in LB starting pH 9.3 unbuffered (pH 9.3), or LB buffered to pH 7.0 with 100 mM HEPES (Buff). (C) ToxR immunoblots of O395 wild-type grown overnight in LB starting pH 7.0 unbuffered at 37°C, pelleted, and resuspended in phosphate buffered saline (PBS) at pH 7.0, pH 8.3, or pH 9.3 for 12 hours.