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. 2015 Apr 7;11(4):e1005145. doi: 10.1371/journal.pgen.1005145

Fig 2. Proteolysis of ToxR is RseP and RpoE-dependent.

Fig 2

(A) ToxR immunoblot of cultures of O395 wild-type or ΔtoxR, ΔrseP or ΔrpoE grown for 48 hours in LB starting pH 7.0 unbuffered (LB), LB starting pH 9.3 unbuffered (pH 9.3), or LB buffered to pH 7.0 with 100 mM HEPES (Buff). (B) Autoagglutination of O395 ΔtcpA, ΔtoxR, wild-type (WT), ΔrseP, ΔrpoE, toxR248, toxR248ΔrseP and toxR248ΔrpoE grown under inducing conditions (LB starting pH 6.5, 30°C) for 15 hours. Autoagglutination can be visualized as a pellet at the bottom of the tube. (C) TcpA and (D) ToxR immunoblots of the cultures in (B).