Table 1. Comparison of hyphal growth rate, conidiation and biomass among mutants and WT of FOC.
Strains | Growth rate (mm/d) a | Conidiation (×106 conidia/mL) | Dry weight (g) d | |
---|---|---|---|---|
Static culture b | Shaken culture c | |||
WT | 11.17 ± 0.01 A e | 2.67 ± 0.33 A | 25.00 ± 7.64 A | 3.17 ± 0.04 A |
ΔFoSlt2 | 6.23 ± 0.01 C | 3.00 ± 0.88 A | 16.67 ± 1.67 A | 2.97 ± 0.02 A |
ΔFoSlt2-c | 11.60 ± 0.02 A | 2.50 ± 0.58 A | 18.33 ± 3.99 A | 3.16 ± 0.16 A |
ΔFoMkk2 | 6.60 ± 0.01 C | 4.00 ± 0.76 A | 20.00 ± 2.89 A | 3.26 ± 0.02 A |
ΔFoMkk2-c | 11.63 ± 0.01 A | 3.33 ± 0.60 A | 26.67 ± 1.67 A | 3.14 ± 0.09 A |
ΔFoBck1 | 7.03 ± 0.01 B | 3.17 ± 0.33 A | 20.00 ± 5.78 A | 3.07 ± 0.15 A |
a Growth rate was detected by measuring the colony diameter of cultures incubated on PDA plates after 6 days at 28°C.
b, c Conidia produced by static and shaking liquid cultures in YPG for 48 h at 28°C and counted with haematocytometer.
d Dry weight of fungal biomass grown in Czapek dox medium.
e Different letters within a column indicate statistically significant differences (P = 0.05). Error bars indicate the standard error from three replicates.