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. 2015 Apr 8;35(14):5549–5556. doi: 10.1523/JNEUROSCI.3614-14.2015

Figure 6.

Figure 6.

Kiss1/Tac2- and Agrp/Npy-expressing neurons are derived from independent subpopulations of Pomc-expressing progenitors. A, Agrp locus in wild-type (WT) and Agrp-CreEGFP mice and immunofluorescent analysis for EGFP in ARC sections of Agrp-CreEGFP mice. Mice were fasted for 30 h to induce CreEGFP expression by activating the Agrp promoter. B, qRT-PCR analysis for Agrp, Pomc, and Kiss1 transcripts in inputs and IPs of Agrp-CreEGFP:RiboTag mice. C, Kiss1 locus in WT and Kiss1-CreEGFP mice and immunofluorescence for EGFP in ARC sections of Kiss1-CreEGFP mice. Mice were gonadectomized to induce CreEGFP expression by activating the Kiss1 promoter. D, qRT-PCR analysis of Kiss1, Tac2, Pomc, and Agrp transcripts in inputs and IPs of Kiss1-CreEGFP:RiboTag mice. Each sample was obtained from a pool of four hypothalamic punches. Graphs show the mean ± SEM. Statistical analysis was performed using two-way ANOVA followed by Bonferroni post-tests. *** p < 0.001. Scale bars: 100 μm.