Figure 9.

A, B, Triplex detection of Grin2a (red), Grin2b (green), and Fos (purple) mRNAs in dorsolateral striatum in the (A) absence or (B) presence of 5 mg/ml RNase treatment. A, Triple labeling for Grin2a, Grin2b, and Fos in the absence of RNase treatment. Fos-positive (white arrows) and Fos-negative (open arrows) cells contained positive signals for both Grin2a and Grin2b mRNAs. The fluorescence channel for DAPI in this image was removed to more clearly observe the particles for each mRNA. B, Triple labeling following prior treatment with 5 mg/ml RNase. Only blue DAPI-labeled DNA was observed. Positive signals were not observed for Grin2a, Grin2b, or Fos.