Table 2.
Fos-positive/Fos-negative ratio gene expression in each context
| Gene | Ratio: Fos-positive/Fos-negativea |
Main effect of Fos cell type | Main effect of Context | Interaction | |
|---|---|---|---|---|---|
| Extinction Context B | Methamphetamine Context A | ||||
| Neuronal marker | |||||
| NeuN | 1.1, n = 9 | 1.2, n = 13 | F(1, 20) = 8.4, p < 0.01 | F(1, 20) = 0.1, p = 0.91 | F(1, 20) = 1.3, p = 0.26 |
| IEGs | |||||
| Fosb | 4.7, n = 7 | 2.5, n = 8 | F(1, 13) = 13, p < 0.01 | F(1, 13) = 0.4, p = 0.55 | F(1, 13) = 0.2, p = 0.67 |
| Total FosB+ΔFosBb | 8.1, n = 3 | 2.6, n = 9 | F(1, 10) = 29, p < 0.01 | F(1, 10) = 0.61, p = 0.45 | F(1, 10) = 4.1, p = 0.07 |
| ΔFosB | 1.0, n = 4 | 4.3, n = 10 | F(1,12) = 1.2, p = 0.29 | F(1, 12) = 0.1, p = 0.82 | F(1, 12) = 1.2, p = 0.29 |
| ΔFosB-2 | 1.7, n = 11 | 0.9, n = 13 | F(1, 22) = 0.9, p = 0.36 | F(1, 22) = 1.6, p = 0.21 | F(1, 22) = 1.2, p = 0.29 |
| Arc | 1.6, n = 10 | 1.2, n = 13 | F(1, 21) = 1.7, p = 0.20 | F(1, 21) = 0.1, p = 0.92 | F(1, 21) = 0.4, p = 0.53 |
| Homer1b | 1.6, n = 10 | 1.3, n = 13 | F(1, 21) = 5.6, p < 0.05 | F(1, 21) = 0.7, p = 0.40 | F(1, 21) = 0.8, p = 0.36 |
| Homer2b | 4.3, n = 7 | 2.0, n = 7 | F(1, 12) = 14, p < 0.01 | F(1, 12) = 3.7, p = 0.07 | F(1, 12) = 4.4, p = 0.06 |
| Receptors | |||||
| Drd1 | 1.3, n = 7 | 1.0, n = 13 | F(1, 18) = 0.5, p = 0.48 | F(1, 18) = 1.2, p = 0.22 | F(1, 18) = 0.7, p = 0.41 |
| Drd2 | 1.0, n = 9 | 1.2, n = 13 | F(1, 20) = 1.6, p = 0.22 | F(1, 20) = 3.9, p = 0.06 | F(1, 20) = 1.9, p = 0.18 |
| Grin1 (GluN1) | 1.0, n = 11 | 1.0, n = 13 | F(1, 22) = 0.2, p = 0.63 | F(1, 22) = 0.6, p = 0.44 | F(1, 22) = 1.9, p = 0.19 |
| Grin2a (GluN2a)b | 1.4, n = 12 | 1.6, n = 17 | F(1, 27) = 13, p < 0.01 | F(1, 27) = 1.2, p = 0.28 | F(1, 27) = 0.5, p = 0.47 |
| Gria1 (GluA1) | 1.0, n = 11 | 1.1, n = 13 | F(1, 22) = 1.5, p = 0.23 | F(1, 22) = 0.1, p = 0.94 | F(1, 22) = 0.5, p = 0.51 |
| Gria2 (GluA2) | 1.2, n = 11 | 1.0, n = 13 | F(1, 22) = 6.1, p < 0.05 | F(1, 22) = 0.5, p = 0.49 | F(1, 22) = 0.1, p = 0.79 |
| Cb1rb | 1.7, n = 12 | 1.4, n = 14 | F(1, 24) = 16, p < 0.01 | F(1, 24) = 0.1, p = 0.96 | F(1, 24) = 1.2, p = 0.28 |
| Trkb1 | 1.6, n = 6 | 1.0, n = 6 | F(1, 10) = 3.0, p = 0.11 | F(1, 10) = 0.1, p = 0.95 | F(1, 10) = 1.0, p = 0.33 |
| Other genes | |||||
| Darpp-32 | 1.3, n = 11 | 1.1, n = 13 | F(1, 22) = 1.7, p = 0.20 | F(1, 22) = 0.8, p = 0.37 | F(1, 22) = 0.3, p = 0.59 |
| Mapk2b | 0.8, n = 8 | 1.2, n = 13 | F(1, 19) = 0.1, p = 0.72 | F(1, 19) = 3.3, p = 0.08 | F(1, 19) = 3.9, p = 0.06 |
| Mecp2_A2 | 0.9, n = 11 | 1.0, n = 13 | F(1, 22) = 0.4, p = 0.55 | F(1, 22) = 0.1, p = 0.86 | F(1, 22) = 0.5, p = 0.49 |
aUsing 2e(−ΔΔCt) values from only matched pairs Fos-positive and Fos-negative within the same animal.
bGenes that also passed the criterion of >1.5 for expression in Fos-positive over Fos-negative neurons.