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. 2015 Mar 12;9(2):111–116. doi: 10.4162/nrp.2015.9.2.111

Fig. 5. Effect of EEIO on expression of various cell cycle-regulating proteins in HT-29 cells.

Fig. 5

Cells were cultured and treated with EEIO for 2 days as described in Fig. 2. Cell lysates were analyzed by immunoblotting with an antibody against p21, p27, p53, CDK2, CDK4, cyclin D1, Rb, pRb, E2F1, or β-actin. A photograph of chemiluminescent detection of a blot, which is representative of 3 independent experiments, is shown.