Fig. 9. Effects of various fractions of EEIO on expression of various cell cycle-regulating proteins in HT-29 cells.
Cells were cultured and treated with various fractions for 2 days as described in Fig. 2. Cell lysates were analyzed by immunoblotting with an antibody against p21, p27, p53, CDK2, CDK4, Cyclin D1, Rb, pRb, E2F1,or β-actin. A photograph of chemiluminescent detection of a blot, which is representative of 3 independent experiments, is shown.