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. 2015 Apr 8;5:9692. doi: 10.1038/srep09692

Figure 2. RBC freezing with novel small molecule IRIs.

Figure 2

(a) The effect of NOGlc (4), β-PMP-Glc (6) and sucrose on post-thaw RBC integrities (n = 2). RBC samples in indicated cryo-solutions were slow-cooled 1°C/min to defined sub-zero temperatures and then rapidly cooled (cooling rate approximately 115°C/min) by immersion into liquid nitrogen and stored at −196°C. (b) The effect of 110 mM β-PMP-Glc (6) on post-thaw RBC integrity using reduced glycerol concentrations (n = 4–30). RBC samples in glycerol solutions with or without 110 mM β-PMP-Glc (6) were slowly cooled (1°C/min) to defined sub-zero temperatures and placed into dry ice and stored at −80°C. All samples were quickly thawed at 37°C and post-thaw hemolysis was quantified post-thaw using Drabkin's assay to determine % RBC integrity (100 - % hemolysis). Error bars represent SEM. Asterisks indicate statistical significance relative to the glycerol control as determined by two-tailed unpaired student's T-test: *, p < 0.05; **, p < 0.01; ***, p < 0.001.