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. 2015 Apr 8;5:9692. doi: 10.1038/srep09692

Figure 3. Two-step cooling profile of β-PMP-Glc.

Figure 3

The effect of 15% glycerol solutions on post-thaw RBC integrity (100 - % hemolysis) with and without 110 mM β-PMP-Glc (6). Samples were slowly cooled (1°C/min) to pre-defined sub-zero temperatures then either immediately thawed (“immediate thaw” curves, dashed lines) or rapidly cooled and stored at −80°C prior to thawing at 37°C (“cooling/storage” curves, solid lines). Results are presented as mean post-thaw RBC integrity (n = 4–30). Error bars indicate standard error of the mean (SEM).